PepPool: CyCMV+RhCMV (TP-UL83+Rh112), scanning

PepPool: CyCMV+RhCMV (TP-UL83+Rh112), scanning

3692-1

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Content

Peptide pool with 133 peptides, 25 ug of each peptide. Supplied in two vials (lyophilized).

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Delivery 4-9 business days

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ELISA substrate: TMB for HRP


Product specifications

Intended use

This peptide pool is intended for studying immune responses in rhesus and cynomolgus macaques against CyCMV TP-UL83 and rhCMV Rh112 using immunoassays such as ELISpot and FluoroSpot. The peptide pool induces cytokine secretion from antigen-specific CD4 and CD8 T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The CyCMV+RhCMV (TP-UL83+Rh112) scanning pool induces secretion of e.g., IFN-γ from monkey T cells. It consists of 133 peptides derived from cynomolgus macaque cytomegalovirus (CyCMV) Tegument phosphoprotein UL83 (Uniprot ID: G8H1B8) and 133 peptides derived from rhesus macaque cytomegalovirus (rhCMV) Rh112 (Uniprot ID: Q772N2). The peptides are 15-mers overlapping with 11 amino acids and cover the complete proteins. The mean purity of the peptides is 83%.

The peptide pool has been validated in IFN-γ ELISpot and FluoroSpot using PBMC from cynomolgus and rhesus macaques.

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Product details

ProductPepPool: CyCMV+RhCMV (TP-UL83+Rh112), scanning
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
ReactivityNon-human primates
Supplied in

Lyophilized

Contents

Peptide pool with 133 peptides, 25 ug of each peptide. Supplied in two vials (lyophilized).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C or below upon receipt. 

PBMC from rhesus macaque (300,000 cells/well) were stimulated with the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ ELISpot. Analysis was done with Mabtech IRIS.
Datasheet/Protocol
SDS

Featured product, Published June 24, 2021

CyCMV + RhCMV peptide pool
Our first peptide pool for non-human primates is released. Will you use it as a positive control in your next ELISpot experiment?
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ELISpot Plus: Monkey IFN-γ (ALP)